-mRNA, 2,2,7-trimethylguanosine, Brr1, Bud13, C, Cwc21, Htz1, Ist3, Isy1, Lea1, Lsm1, Mud2, Nam8, Pat1, RNA, Rpn4, Soh1, Spt3, Srb2, Swr1, TMG, Tgs1, Trf4, Trimethylguanosine, Trimethylguanosine synthase, amino acids, budding, guanine, human, human Tgs1, human Tgs1 guanine-N2 methyltransferase, m(7)G, mammalian somatic cells, mud2Delta, nam8Delta, nonessential genes, small nuclear ribonucleoprotein, spliceosomal small nuclear RNAs, tgs1Delta, trimethylguanosine, wild-type TGS1, yeast, yeast and human cap trimethylguanosine synthase