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Author Details

F W Studier
Brookhaven National Laboratory
1963
103
63
Andrej Sali (CM4AI)
PMIDPaper TitleJournal TitlePublished Year
30016582T7 Expression Systems for Inducible Production of Proteins from Cloned Genes in E. coli.Curr Protoc Mol Biol2018
26153419Recombinant transfer in the basic genome of Escherichia coli.Proc Natl Acad Sci U S A2015
24203322Stable expression clones and auto-induction for protein production in E. coli.Methods Mol Biol2014
23323273In memoriam: John Dunn, scientist extraordinaire (May 29, 1944-July 13, 2012): reminiscences of John's 40 years of exhilarating scientific research at BNL.Protein Expr Purif2013
19786035Genome sequences of Escherichia coli B strains REL606 and BL21(DE3).J Mol Biol2009
19765592Understanding the differences between genome sequences of Escherichia coli B strains REL606 and BL21(DE3) and comparison of the E. coli B and K-12 genomes.J Mol Biol2009
19765591Tracing ancestors and relatives of Escherichia coli B, and the derivation of B strains REL606 and BL21(DE3).J Mol Biol2009
18235434Protein production and purification.Nat Methods2008
18707125Structural characterization and reversal of the natural organophosphate resistance of a D-type esterase, Saccharomyces cerevisiae S-formylglutathione hydrolase.Biochemistry2008
16455797Structures of wild-type and mutant human spermidine/spermine N1-acetyltransferase, a potential therapeutic drug target.Proc Natl Acad Sci U S A2006
15722447Structure and mechanism of ADP-ribose-1''-monophosphatase (Appr-1''-pase), a ubiquitous cellular processing enzyme.Protein Sci2005
16211523New York-Structural GenomiX Research Consortium (NYSGXRC): a large scale center for the protein structure initiative.J Struct Funct Genomics2005
15915565Protein production by auto-induction in high density shaking cultures.Protein Expr Purif2005
15223315Multiple roles of T7 RNA polymerase and T7 lysozyme during bacteriophage T7 infection.J Mol Biol2004
12499548Structure of a yeast hypothetical protein selected by a structural genomics approach.Acta Crystallogr D Biol Crystallogr2003
12833551Crystal structure of a putative CN hydrolase from yeast.Proteins2003
12112709X-ray structure of Saccharomyces cerevisiae homologous mitochondrial matrix factor 1 (Hmf1).Proteins2002
11698677Structural genomics of enzymes involved in sterol/isoprenoid biosynthesis.Proc Natl Acad Sci U S A2001
11116182Slab-gel electrophoresis.Trends Biochem Sci2000
11271477Formation of concentration gradient and its application to DNA capillary electrophoresis.Electrophoresis2000
10508510Structural genomics: beyond the human genome project.Nat Genet1999
9694291Multi-capillary optical waveguides for DNA sequencing.Electrophoresis1998
9654445Pausing and termination by bacteriophage T7 RNA polymerase.J Mol Biol1998
9192997Mechanism of inhibition of bacteriophage T7 RNA polymerase by T7 lysozyme.J Mol Biol1997
9192998Mutant bacteriophage T7 RNA polymerases with altered termination properties.J Mol Biol1997
8627626Assembly of T7 capsids from independently expressed and purified head protein and scaffolding protein.J Mol Biol1996
8900164Biochemical analysis of mutant T7 primase/helicase proteins defective in DNA binding, nucleotide hydrolysis, and the coupling of hydrolysis with DNA unwinding.J Biol Chem1996
8900163Selection, identification, and genetic analysis of random mutants in the cloned primase/helicase gene of bacteriophage T7.J Biol Chem1996
8832178Polyacrylamide solutions for DNA sequencing by capillary electrophoresis: mesh sizes, separation and dispersion.Electrophoresis1996
8627627Purification and characterization of T7 head-tail connectors expressed from the cloned gene.J Mol Biol1996
7608967Isolation of transcriptionally active mutants of T7 RNA polymerase that do not support phage growth.J Mol Biol1995
8572293Ligation of hexamers on hexamer templates to produce primers for cycle sequencing or the polymerase chain reaction.Anal Biochem1995
7844820Consecutive low-usage leucine codons block translation only when near the 5' end of a message in Escherichia coli.J Mol Biol1995
8171031The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase.Proc Natl Acad Sci U S A1994
7678594Effects of consecutive AGG codons on translation in Escherichia coli, demonstrated with a versatile codon test system.J Bacteriol1993
1321823Cloning and expression of gene 4 of bacteriophage T7 and creation and analysis of T7 mutants lacking the 4A primase/helicase or the 4B helicase.J Biol Chem1992
1465615DNA sequencing by primer walking with strings of contiguous hexamers.Science1992
1321824Large scale purification and biochemical characterization of T7 primase/helicase proteins. Evidence for homodimer and heterodimer formation.J Biol Chem1992
2023259Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system.J Mol Biol1991
1990002Resolution of branched DNA substrates by T7 endonuclease I and its inhibition.J Biol Chem1991
1902522Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor.J Mol Biol1991
2023261Creation of a T7 autogene. Cloning and expression of the gene for bacteriophage T7 RNA polymerase under control of its cognate promoter.J Mol Biol1991
2199796Use of T7 RNA polymerase to direct expression of cloned genes.Methods Enzymol1990
2170355Characterization of a bimobile DNA junction.J Biol Chem1990
2403641Signal-mediated import of bacteriophage T7 RNA polymerase into the Saccharomyces cerevisiae nucleus and specific transcription of target genes.Mol Cell Biol1990
2780548A strategy for high-volume sequencing of cosmid DNAs: random and directed priming with a library of oligonucleotides.Proc Natl Acad Sci U S A1989
3065146Targeting bacteriophage T7 RNA polymerase to the mammalian cell nucleus.Gene1988
3259634Molecular substructure of a viral receptor-recognition protein. The gp17 tail-fiber of bacteriophage T7.J Mol Biol1988
2842783Expression of the bisphosphatase domain of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in Escherichia coli.Proc Natl Acad Sci U S A1988
2838843Model for how type I restriction enzymes select cleavage sites in DNA.Proc Natl Acad Sci U S A1988
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Collaborators

Brookhaven National Laboratory
Co-authored papers 27
Brookhaven National Laboratory
Co-authored papers 7
The State University of New Jersey
Co-authored papers 5
Albert Einstein College of Medicine
Co-authored papers 5
University of California San Francisco
Co-authored papers 4
Brookhaven National Laboratory
Co-authored papers 4
Brookhaven National Laboratory
Co-authored papers 4
Brookhaven National Laboratory
Co-authored papers 3
Case Western Reserve University
Co-authored papers 3
Albert Einstein College of Medicine
Co-authored papers 3
Brookhaven National Laboratory
Co-authored papers 3
Albert Einstein College of Medicine
Co-authored papers 2
NYU Langone Hospital - Brooklyn Brooklyn
Co-authored papers 2
University of Illinois Urbana-Champaign
Co-authored papers 2
Co-authored papers 2
Eli Lilly and Co.
Co-authored papers 2
Scripps Institution of Oceanography, University of California San Diego
Co-authored papers 2
School of Public Health, Capital Medical University
Co-authored papers 1
Dana-Farber Cancer Institute
Co-authored papers 1
The University of Texas MD Anderson Cancer Center
Co-authored papers 1
Center for Genomics and Systems Biology, New York University Abu Dhabi (NYUAD)
Co-authored papers 1
National Institutes of Health
Co-authored papers 1
Robert Wood Johnson Medical School, Rutgers University
Co-authored papers 1
Rensselaer Polytechnic Institute
Co-authored papers 1
Co-authored papers 1
Structural Genomics Consortium Karolinska Institute Stockholm Sweden.
Co-authored papers 1
Nankai University
Co-authored papers 1
Co-authored papers 1
The Scripps Research Institute, USA The Skaggs Institute for Chemical Biology
Co-authored papers 1
Co-authored papers 1